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KMID : 0613820060160050870
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Journal of Life Science
2006 Volume.16 No. 5 p.870 ~ p.875
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Whitening Effect of Mycelial Culture Broth of Paecilomyces japonica in the Mixture of Cucumber and Grape Extracts
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Lee Young-Hoon
Park Sang-Sang
Lee Sang-Won
Lee Sung-Ho
Park Ki-Hoon
Choi Young-Ju
Gal Sang-Wan
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Abstract
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This study was carried out to investigate the whitening effect of mycelial culture broth of P. japonica in the mixture of cucumber and grape extracts. In the inhibition test of melanin biosynthesis of melanoma cell, B16BL6, the culture broth of P. japonica more then 50 μl/ml (5%) concentration inhibited the melanin biosynthesis of the cell entirely without cytotoxicity. More then 10 days incubation of P. japonica in the mixture was required to have the inhibition activity. In vitro inhibition test of melanin biosynthesis of the culture broth of P. japonica was investigated in the concentration dependent manner of 10% to 50%. 30% concentration of the culture broth inhibited completely tyrosinase activity. In the cytotoxicity test, cucumber and grape extract itself has a strong cytotoxicity to the melanoma cell, B16Bl6. The value of IC?? of the cucumber and grape extracts against the melanoma cells was 5% concentration. However, the culture broth of P. japonica incubated in the cucumber and grape extracts did not show the cytotoxcity up to 20% against melanoma cell, B16BL6. Therefore, we concluded that the culture broth of P. japonica in the mixture of cucumber and grape extracts can be used as a whitening cosmetic resource.
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KEYWORD
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Paecilomyces japonica, whitening, B16BL6 mouse melanoma cell, melanin biosynthesis, tyrosinase inhibitor
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